eBioscience produces premium-quality yet value-priced immunological reagents that give you optimal signal-to-noise in your experiments. Our product expertise includes commonly used immunophenotyping markers (mouse, human and rat CDs), cytoskeletal and cytoplasmic proteins, chemokine receptors, as well as intracellular cytokines/chemokines and intra-nuclear proteins including transcription factors.
Over 10,000 products are listed in our eCatalog along with the technical information and a large selection of flexible sizes (10 μg to 500 μg) and conjugates for each antibody. eBioscience was established in 1999 in San Diego by antibody experts whom you have trusted for many years.
Please browse through our eCatalog or find specific products using the search box on the top right of each web page. To request our print literature, click here.
Below are protocols for our products which we have optimized to support their use. If we can be of further assistance, please contact eBioscience Tech Support. Thank you for visiting eBioscience.
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Protocol A: Cell Preparation of Tissue Culture Cells
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Protocol B: Cell Preparation from Lymphoid Tissue
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Protocol C: Cell Preparation from Non-Lymphoid Tissue
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Protocol D: Isolation of PBMC from Whole Blood
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Protocol A: RBC Lysis of Mouse Splenocytes
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Protocol B: RBC Lysis of Mouse Blood
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Protocol C: RBC Lysis of Human Peripheral Blood
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Protocol D: Lysis of Human Blood for Flow Cytometric Analysis
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Protocol A: Cell Suspensions
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Protocol B: Human Lysed Whole Blood
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Protocol A: Two step protocol for intracellular (cytoplasmic) proteins
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Protocol B: One step protocol for intracellular proteins
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Protocol A: Staining Dead Cells with Propidium Iodide or 7-AAD
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Protocol B: Staining Live Cells with Calcein Dyes
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Protocol C: Staining Dead Cells with eBioscience Fixable Viability eFluor® Dyes
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Protocol A: ELISA Protocol using Ready-Set-Go! Set
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Protocol B: ELISA Protocol using Ready-Set-Go! Kits (with pre-coated plates)
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Protocol C: ELISA Protocol using Antibody Pairs
Measurement of biological activity of cytokines and their concentrations by cellular proliferation of primary cell cultures or isolated cell lines that are dependent and/or responsive to a specific growth factor.
Protocols describing in vitro bioassays using neutralizing anti-mouse and anti-human cytokine antibodies.
Antibodies used for in vivo studies where the injected antibody is intended to either deplete a specific cell type or induce activation of cells. eBioscience offers monoclonal antibodies to mouse cell surface antigens and cytokines for in vitro or in vivo functional studies.