Staining of unstimulated (red histogram) or LPS- and Anti-Mouse CD40-stimulated (cat. 16-0401) (purple histogram) mouse splenocytes with 0.125 µg of Anti-Mouse CD70 PerCP-eFluor® 710. Total viable cells were used for analysis.
Contents: Anti-Mouse CD70 PerCP-eFluor® 710 Catalog Number: 46-0701 Concentration: 0.2 mg/mL Formulation: aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: FR70 Host/Isotype: Rat IgG2b, κ
Product Options
Cat. No.
Size
Price
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46-0701-80
25 ug
46-0701-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The FR70 monoclonal antibody reacts with mouse CD70, a 30-33 kDa type II transmembrane glycoprotein. CD70 is a member of the TNF family and is expressed by activated mouse T and B lymphocytes. Interaction of CD70 with CD27, its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.
Applications Reported
This FR70 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This FR70 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.
Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.
Oshima, H., H. Nakano, et al. (1998). Characterization of murine CD70 by molecular cloning and mAb Int Immunol 10(4): 517-26.
\Akiba, H., H. Oshima, et al. (1999). CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells. J Immunol 162(12): 7058-66.