Staining of normal human peripheral blood cells with Mouse IgG1 κ Isotype Control PE (cat. 12-4714) (open histogram) or Anti-Human CD27 PE (filled histogram). Cells in the lymphocyte gate were used for analysis.
Contents: Anti-Human CD27 PE Catalog Number: 12-0279 Concentration: Suffix -71/73, 20 μL (0.25 μg)/test; Suffix -41/42, 5 μL (0.25 μg)/test Formulation: aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: O323 Host/Isotype: Mouse IgG1, κ HLDA Workshop: IV T-186
Product Options
Cat. No.
Size
Price
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12-0279-71
25 tests
12-0279-73
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŪ 647 conjugated products with eFluorŪ 660 conjugated products.
Description
The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction.
Applications Reported
The O323 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The O323 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. Refer to catalog number suffix on the vial for amount to use per test: 71/73 are 20 μL (0.25 μg) per test; whereas 41/42 are 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.