Surface staining of LPS and Anti-Mouse/Rat CD40 Functional Grade Purified (cat. 16-0402) stimulated mouse splenocytes with Anti-Mouse CD70 PE (cat. \It{12-0701}). Autofluorescence is shown via open histogram. Total viable cells were used for analysis.
Contents: Anti-Mouse CD70 Purified Catalog Number: 14-0701 Concentration: 0.5 mg/ml Formulation: aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer Storage Conditions: Store at 2-8°C. Clone: FR70 Host/Isotype: Rat IgG2b, κ
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Size
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14-0701-81
50 ug
14-0701-82
100 ug
14-0701-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The FR70 monoclonal antibody reacts with mouse CD70, a 30-33 kDa type II transmembrane glycoprotein. CD70 is a member of the TNF family and is expressed by activated mouse T and B lymphocytes. Interaction of CD70 with CD27, its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.
Applications Reported
The FR70 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. FR70 has also been reported in blocking of CD70 in vitro. (Please use Functional Grade purified FR70, cat. 16-0701, in functional assays.)
Applications Tested
The FR70 antibody has been tested by flow cytometric analysis of mouse LPS-activated splenocyte suspensions. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Oshima, H., H. Nakano, et al. (1998). "Characterization of murine CD70 by molecular cloning and mAb." Int Immunol 10(4): 517-26. Akiba, H., H. Oshima, et al. (1999). "CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells." J Immunol 162(12): 7058-66.