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High Sensitivity ELISA Kits
Low abundance target analysis made easy.
> High Sensitivity ELISA Products
High Sensitivity ELISA Kits enable the accurate quantification of extremely low concentrations (< 1 pg/ml) of protein / target analytes
from serum, plasma or cell culture sample.
Advantages of High Sensitivity ELISA Kits
- Sensitive – amplify low cytokine concentrations for reliable quantification at < 1 pg/ml.
- Maximizes resources – 50 µl sample volume requirement is ideal for limited sample resources.
- Easy-to-use – straight-forward protocol with all reagents included.
The High Sensitivity ELISA Protocol:
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Assay set up is similar to a conventional ELISA, using Biotin-streptavidin-HRP, but includes the addition of an amplification reagent I, which contains biotinyl-tyramide.
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Horseradish peroxidase (HRP) converts multiple biotinyl-tyramide molecules into highly reactive derivatives (free radicals). These bind covalently to any
protein in the well, e.g. blocking proteins. Thus the amount of reacted biotinyl-tyramide is proportional to the amount of HRP in the well.
Excess biotinyl-tyramide is washed out.
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Amplification reagent II contains Streptavidin-HRP, which binds to the biotin sites created during the biotinyl-tyramide reaction, thus multiplying the HRP molecules
available at the surface for the substrate reaction.
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High Sensitivity ELISAs are based on a patent from PerkinElmer LAS, patent number 5,731,158; 5,583,001; 5,196,306.
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