 |
eFluorŪ Nanocrystal Conjugation Kits
|
These unique kits allow you to combine the power of Nanocrystals with your own antibody or protein of interest,
enabling their use in multiple fluorescence applications such as flow cytometry and immunofluorescence microscopy.
Key features of eBioscience eFluorŪ Nanocrystal Conjugation Kits include:
- Easy to perform - optimized kits deliver a complete, simple protocol for protein conjugation.
- Fast - under 30 minutes "hands on" time. Compare with other protein conjugation kits requiring > 3 hrs.
- Consistent results - all key reagents are included, giving you dependable results every time.
- Flexible - offered in two chemistries, Amine-Reactive and Sulfhydryl-Reactive, for optimal labeling of your protein, monoclonal antibody, or polyclonal antibody.
|
eFluorŪ Nanocrystal Conjugation Kits
|
| Product |
Cat. No. |
Fluorophore |
Application Notes |
Size |
| eFluorŪ Nanocrystal Conjugation Kit - Amine Reactive |
|
Conjugation to biomolecules containing primary amines (-NH2)
Use labeled products for Immunocytochemistry, Immunohistochemistry, or Flow Cytometry
|
Suitable for performing two protein / antibody conjugations
|
| eFluorŪ Nanocrystal Conjugation Kit - Sulfhydryl Reactive |
|
Conjugation to biomolecules containing thiol, cysteine, or disulfide groups
Use labeled products for Immunocytochemistry, Immunohistochemistry, or Flow Cytometry
|
Suitable for performing two protein / antibody conjugations
|
|
|
|
|
eFluorŪ Nanocrystal Conjugation of Monoclonal Antibodies.
MCF-7 cells were stained using Anti-Cytokeratin (AE1) eFluorŪ 605NC (pseudo-colored blue) and Anti-Tubulin (DM1A) eFluorŪ 650NC (green) reagents conjugated using using eFluorŪ Nanocrystal Conjugation Kit - Sulfhydryl Reactive. Anti-Ki67 (20Raj1) Alexa FluorŪ 647 is shown in red.
|
|
eFluorŪ Nanocrystal Conjugation of Polyclonal Antibodies.
MDCK cells stained with polyclonal Rabbit Anti-Histone H3 eFluorŪ 605NC (green) conjugated using eFluorŪ Nanocrystal Conjugation Kit - Amine Reactive. Anti-Tubulin (DM1A) eFluorŪ 650NC is shown in red.
|
Two high-performance options for nanocrystal conjugation allow you to choose the kit best suited for your antibody or protein target. These kits are designed for conjugation to either free amine (-NH2) or to sulfhydryl (thiol) groups, either of which may be present in your protein of interest. For additional details about choosing the kit which is right for you, view our technical tips and protocols or contact our experienced technical support scientists.
 Top
- Conjugate eFluorŪ Nanocrystals to proteins containing sulfhydryl (thiol), cysteine, or disulfide groups
- Suitable for both monoclonal and purified polyclonal antibody conjugation as well as other proteins (MW between 30-150 kDa), and nucleic acids
- Use labeled products for Immunocytochemistry, Immunohistochemistry, or Flow Cytometry
 |
eFluorŪ Nanocrystals for Immunohistochemistry on frozen tissue.
Frozen mouse spleen stained with Anti-CD11b (M1/70) eFluorŪ 650NC (red), conjugated using eFluorŪ Nanocrystal Conjugation Kit -
Sulfhydryl Reactive. Anti-CD45R (B220) Alexa FluorŪ 488 staining shown in green.
|
Top
- Conjugate eFluorŪ Nanocrystals to proteins containing primary amines (-NH2)
- Suitable for both monoclonal and purified polyclonal antibody conjugation as well as other proteins (MW between 30-150 kDa), and nucleic acids
- Use labeled products for Immunocytochemistry, Immunohistochemistry, or Flow Cytometry
 |
eFluorŪ Nanocrystals for Immunohistochemistry on formalin-fixed,
paraffin-embedded tissue (FFPE).
Staining of a lymphoid follicle in FFPE human tonsil tissue using Anti-CD20 (L26) eFluorŪ 605NC reagent conjugated using eFluorŪ Nanocrystal
Conjugation Kit - Amine Reactive.
|
 |
eFluorŪ Nanocrystals for Flow Cytometry
Mouse splenocytes were stained with anti-CD4 (GK1.5) conjugated to eFluorŪ 605NC using the eFluorŪ Nanocrystal Conjugation Kit - Amine Reactive
and anti-CD8 (53-6.7) eFluorŪ 660. Cells in the lymphocyte gate are shown.
|
Top
eFluorŪ Nanocrystal Conjugation Kit Amine-Reactive Protocol
eFluorŪ Nanocrystal Conjugation Kit Sulfhydryl-Reactive Protocol
|
I. eFluorŪ Nanocrystal Conjugation
|
II. Quenching the Reaction
|
III. Purification of the eFluorŪ Nanocrystal Conjugate
Hands on time: < 15 min / Total protocol: < 4 hr
Top
|