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Quantify up to 20 targets from one sample
Multiple analyte detection is an invaluable tool for the comprehensive study of biological systems comprised of networks of cytokines, chemokines, growth factors and related proteins. The FlowCytomix™ Multiple Analyte Detection System, developed by Bender MedSystems, is designed to simultaneously quantify up to 20 protein targets quickly and easily.
Advantages of the FlowCytomix™ Multiple Analyte Detection System
- Sample friendly – requires only 25 μl samples from serum, plasma, or cell culture supernatants.
- Easy to customize – choose protein targets to create your own assay panels.
- Flexible protocol – use with 96 well plates or individual tubes.
- Free software – save hundreds of dollars on data analysis software.
- Broad usage – compatible with most flow cytometers, including single laser instruments.
How FlowCytomix™ Works
FlowCytomix™ Kits contain fluorescent bead sets, each pre-coated with a unique antibody specificity.
Within two bead size populations, A and B (4 μm & 5 μm), there are multiple bead subsets, differentiated by varying intensities of an internal fluorescent dye. When the FlowCytomix™ dye is excited by an Argon, He-Ne, or UV laser, the dye emits at 690 nm (the far red spectrum), and is detected in the FL-3/FL-4 channel. The combination of two different bead sizes and different internal dye intensities makes it possible to distinguish up to 20 bead sets in one fluorescent channel. Streptavidin-PE, which binds to the biotin conjugate, emits at 578 nm and is detected in the FL-2 channel, allowing the quantification of the analyte.
FlowCytomix™ bead-based assays follow same principles as a sandwich immunoassay:
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