Stage 1: Single-cell subcloning of hybridomas
Stage 2: Antibody purity check
Stage 3: Antibody conjugation
- For flow cytometry reagents, fluorochrome-to-antibody ratios are optimized for each clone to achieve the best signal-to-noise.
Stage 4: Post-bottling quality control
Stage 5: FG purified antibodies, recommended for bioassays and in vivo studies, are further tested for sterility and endotoxin level.
Stage 6: Final products are tested for their performance in the most common applications including surface and intracellular flow cytometry, ELISA, and when appropriate in functional assays (e.g., in vitro activation or neutralization). In addition, selected products are tested by immunohistochemical staining of frozen tissues, immunoprecipitation, and immunoblotting.