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Best ProtocolsŪ |
eBioscience produces premium-quality yet value-priced immunological reagents that give you optimal signal-to-noise in your experiments. Our product expertise includes commonly used immunophenotyping markers (mouse, human and rat CDs), cytoskeletal and cytoplasmic proteins, chemokine receptors, as well as intracellular cytokines/chemokines and intra-nuclear proteins including transcription factors. FLOW CYTOMETRY:Cell Preparation for Flow Cytometry (Click Here to Download PDF)
Red Blood Cell Lysis (Click Here to Download PDF)
Staining Cell Surface Antigens for Flow Cytometry (Click Here to Download PDF)
Staining Intracellular Antigens for Flow Cytometry
Viability Staining (Click Here to Download PDF)
Annexin V Kit for Detection of Early Apoptotic Events (Click Here to Download PDF)
Pharmacological Induction of Apoptosis With Camptothecin (Click Here to Download PDF)
IMMUNOASSAY:Enzyme Linked Immunosorbent Assay (ELISA)
ELISPOT Ready-SET-Go! Protocol
BIOASSAY:Bioassays, CytokinesMeasurement of biological activity of cytokines and their concentrations by cellular proliferation of primary cell cultures or isolated cell lines that are dependent and/or responsive to a specific growth factor. Cytokine Neutralizing ProtocolsProtocols describing in vitro bioassays using neutralizing anti-mouse and anti-human cytokine antibodies. In Vivo use of AntibodiesAntibodies used for in vivo studies where the injected antibody is intended to either deplete a specific cell type or induce activation of cells. eBioscience offers monoclonal antibodies to mouse cell surface antigens and cytokines for in vitro or in vivo functional studies.
T Cell Activation using anti-CD3 mAb
WESTERN BLOT (WB)/IMMUNOPRECIPITATION (IP):
Western Blotting (Immunoblotting) (Click Here to Download PDF)
Immunoprecipitation (Click Here to Download PDF)
IMMUNOHISTOCHEMISTRY (IHC)/IMMUNOCYTOCHEMISTRY (ICC):
Immunofluorescent Staining of Intracellular Antigens on Cultured Cells (Click Here to Download PDF)
Immunofluorescent Staining of Formalin-Fixed Paraffin-Embedded Tissues (Click Here to Download PDF)
eFluor® Nanocrystals for Immunofluorescent Staining of Frozen Tissue Sections (Click Here to Download PDF)
eFluor® Nanocrystals:
eFluor® Nanocrystals In Vivo Imaging (Click Here to Download PDF)
eFluor® Nanocrystals Cell Labeling (Click Here to Download PDF)
eFluor® Nanocrystals (Reactive) Conjugation (Click Here to Download PDF)
eFluor® Nanocrystal Conjugation Kits:
eFluor® Nanocrystal Conjugation Kit Amine-Reactive Protocol (Click here to Download PDF)
eFluor® Nanocrystal Conjugation Kit Sulfhydryl-Reactive Protocol (Click here to Download PDF)
eFluor® Nanocrystal Conjugation Kits - Immunofluorescent Staining of Intracellular Antigens on Cultured Cells (Click here to Download PDF)
eFluor® Nanocrystal Conjugation Kits - Immunofluorescent Staining of FFPE Tissue Sections (Click here to Download PDF)
eFluor® Nanocrystal Conjugation Kits - Immunofluorescent Staining of Frozen Tissue Sections (Click here to Download PDF)
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