Contents: Mouse IL-4 Recombinant Protein Catalog Number: 14-8041 Formulation: 20mM Phosphate, 0.38M NaCl, pH 6.5 and 1.0% BSA as a carrier. 0.22 uM filtered. Storage Conditions: Store at less than or equal to -70°C. Handling Conditions: For best recovery, quick-spin vial prior to opening. Use in a sterile environment Source: E. coli expressed amino acids his 21-ser140 of mature mouse IL-4 (accession # NM_021283). Molecular Mass: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 13,557. The DTT reduced protein migrates as a 13 kDa polypeptide on SDS-PAGE. Purity: Greater than 98%, as determined by SDS-PAGE Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Bioactivity: The ED50 of this protein, as measured by CTLL-2 proliferation assay, is 100-300 pg/ml, corresponding to a specific activity of 1x107-3.3x106 Units/mg.
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14-8041-62
5 ug
14-8041-80
25 ug
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Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Recombinant mouse IL-4 is biologically active and can promote proliferation of mouse T lymphocytes in culture. It is also used as a cytokine standard in mouse IL-4 ELISA.
Applications Tested
This reagent has been tested by sandwich ELISA using the anti-mouse IL-4 antibody pair (11B11/BVD6-24G2) and in bioassays using the mouse cell line CTLL-2. The ED50 measured in a CTLL-2 proliferation assay is typically 100-300 pg/ml, corresponding to a specific activity of approximately 1x107-3.3x106 Units/mg.