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  eFluorŽ Organic Dyes
eFluorŽ Organic Dyes
  Flow Cytometry
 
All eFluor® reagents are named for their emission wavelength to provide consistent and logical designation.
 
 
eFluor® 450
  eFluor® 450 Replaces Pacific Blue®
  Comparison of 450 nm Emitting Dyes
  eFluor® 450 Emission Spectra
 
eFluor® 660
  eFluor® 660 Replaces Alexa Fluor® 647
  eFluor® 660 for Fluorescent Microscopy
  eFluor® 660 Emission Spectra
 
PerCP-eFluor® 710
Attention: Replacement of all PE-Cy5.5 products with PerCP-eFluor® 710 or PerCP-Cy5.5
  PerCP-eFluor® 710 Emission Spectra
  Comparison of PerCP-eFluor® 710 and PerCP-Cy5.5 Conjugates
  Stability of PerCP-eFluor® 710 Tandem Dye Conjugates
 
APC-eFluor® 780
  APC-eFluor® 780 Replaces APC-Alexa Fluor® 750
  Comparison with Competitor Product
  APC-eFluor® 780 Emission Spectra
   
 
 
eFluor® 450
 
eFluor® 450 organic dye has been developed to provide a high performance fluorochrome for multicolor flow cytometry. It is designed for use with instruments equipped with a violet laser and exhibits an emission peak at 450 nm. Extensive characterization indicates that eFluor® 450 is
 
equivalent or better than our current Pacific Blue® reagent and other 450 nm emitting dyes in fluorescence intensity, stability, and overall performance and therefore will replace Pacific Blue® in our product portfolio.
 

eFluor® 450 Replaces Pacific Blue®
To view more data comparing eFluor® 450 with Pacific Blue® click here.
 
  eFluorŽ 450 vs Pacific BlueŽ
 
 

Performance comparison with Pacific Blue®
 
Human PBMCs were stained with anti-CD45RA clone HI100 conjugated
to eFluor® 450 (left panel) or Pacific Blue® (middle panel). The right
panel shows the overlay of both histograms.
 
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  Organic Dyes Products
 
Comparison with other 450 nm Emitting Dyes
 
  eFluorŽ 450 vs V450 and VioBlue  
Comparison data with Horizon V450 and VioBlue
LEFT:  Mouse splenocytes were stained with anti-CD19 (clone 1D3) conjugated to eFluor® 450 (pink-solid line) or Horizon V450 (green-dashed line).
RIGHT:  Mouse splenocytes were stained with anti-CD8 (clone 53-6.7) conjugated to eFluor® 450 ( pink-solid line) or VioBlue (purple-dashed line).
 
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  Organic Dyes Products
 
eFluor® 450 Emission Spectra
 
  eFluorŽ 450 Emission Spectra
 
 

eFluor® 450 Organic Dye
 
Emission characteristics of an eFluor® 450 conjugated antibody with the
recommended band pass filter (450/50) for this fluorochrome.
 
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  Organic Dyes Products
 
 
eFluor® 660
  eFluor® 660 organic dye has been developed to provide a high performance fluorochrome for multicolor flow cytometry. It is designed for use with instruments equipped with a red laser and exhibits an emission peak at 660 nm. Extensive evaluation of eFluorŽ 660 shows that its fluorescent performance is equivalent or better than our current Alexa Fluor® 647 reagent.
 

eFluor® 660 Replaces Alexa Fluor® 647
To view more data comparing eFluor® 660 with Alexa Fluor® 647 click here.
 
  eFluor 660  
 
The MDCK cell line was stained with rat IgG1 isotype control (dotted line) or the anti-CD324 (E-cadherin) clone DECMA-1 conjugated to eFluor 660 (red histogram) or Alexa Fluor 647 (gray histogram).
 
  eFluor 660  
 
Human peripheral blood monocytes were stained with mouse IgM isotype control (dotted line) or the anti-CD36 clone NL07 conjugated to eFluor 660 (red histogram) or Alexa Fluor 647 (gray histogram).
 
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  Organic Dyes Products
 
 
eFluor 660® for Fluorescent Microscopy
 
  eFluor 660  
 
Immunofluorescent image of cryosection of mouse intestine stained with eFluor 660 conjugated anti-mouse LYVE-1 antibody and nuclear counterstain DAPI.
 
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  Organic Dyes Products
 
eFluor® 660 Emission Spectra
 
  eFluor 660 emission spectra  
eFluor 660® Organic Dye
Emission characteristics of an eFluor 660 conjugated antibody with the recommended bandpass filter (660/20) for this fluorochrome.
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  Organic Dyes Products
 
 
PerCP-eFluor® 710
  Attention: Replacement of all PE-Cy5.5 products with PerCP-eFluor® 710 or PerCP-Cy5.5
  The PerCP-eFluor® 710 tandem dye has been developed as an alternative choice for the FL3 channel on a blue (488 nm) laser line. PerCP-eFluor® 710 is consistently brighter than PerCP-Cy5.5 when evaluated in clone to clone comparison tests on identical tissue samples. Although PerCP-eFluor® 710 is slightly red-shifted in its emission compared to PerCP-Cy5.5, it uses the same filter sets as those recommended for PerCP-Cy5.5 detection thereby requiring no changes to instrumentation for customers currently using PerCP-Cy5.5 or PE-Cy5.5 tandem dye conjugates. The PerCP-eFluor® 710 tandem dye is very stable and testing indicates that exposure to ambient light and standard fixation protocols have minimal effects on compensation requirements for this fluorochrome.
 

PerCP-eFluor® 710 Emission Spectra
 
  PerCP-eFluorŽ 710 Emission Spectra  
Emission characteristics of PerCP-tandem dye conjugates

The emission spectra of a PerCP-eFluor® 710 (red) conjugated antibody and a PerCP-Cy5.5 (pink) conjugated antibody are overlayed. For both dyes, a 685 nm dichroic mirror is recommended along with a 695/40 or 710/40 band pass filter.
 
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  Organic Dyes Products
 
Comparison of PerCP-eFluor® 710 and PerCP-Cy5.5 Conjugates
 
  PerCP-eFluorŽ 710 vs PerCP-Cy5.5
 
PerCP-eFluor® 710 is consistently brighter than PerCP-Cy5.5 in a clone to clone comparison
 
Anti-mouse CD4 (clone RM4-5), anti-mouse CD8 (clone 53-6.7), and anti-human CD3 (clone OKT3) were conjugated to either PerCP-eFluor® 710 (red) or PerCP-Cy5.5 (pink) for direct comparison. Mouse splenocytes were stained with anti-CD4 (left panel) or anti-CD8 (middle panel). Human PBMCs were stained with anti-CD3 (right panel).
 
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  Organic Dyes Products
 
Stability of PerCP-eFluor® 710 Tandem Dye Conjugates
 
  PerCP-eFluorŽ 710 Stability  
PerCP-eFluorŽ 710 Compensation Matrix

PerCP-eFluor® 710 is minimally affected by fixation or exposure to light.

Human PBMCs were stained with anti-CD4 PerCP-eFluor® 710 and analyzed immediately (red). The sample was then divided and either exposed to ambient light for 6 hours on the benchtop (blue) or fixed in 2% paraformaldehyde overnight at 4°C (green) and analyzed again. The compensation and histogram data demonstrate that these treatments have minimal effects on brightness or compensation requirements for the PerCP-eFluor® 710 tandem dye.
 
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  Organic Dyes Products
 
 
APC-eFluor® 780
 
APC-eFluor® 780 tandem dye is a high performance fluorochrome for multicolor flow cytometry. APC-eFluor® 780 is optimally excited by the red laser (633 nm) and has an emission peak of 780 nm. With similar spectral properties to the APC-Alexa Fluor® 750 tandem dye, APC-eFluor® 780 requires no changes to instrument configuration for use.
 
The APC-eFluor® 780 tandem dye can be easily compensated when used with APC and thus offers a quality reagent for use in multicolor staining panels. APC-eFluor® 780 will be replacing APC-Alexa Fluor® 750 in our product portfolio.
 

APC-eFluor® 780 replaces APC-Alexa Fluor® 750
To view more data comparing eFluor® 780 with APC-Alexa Fluor® 750 click here..
 
  APC-eFluorŽ 780 vs APC-Alexa FluorŽ 750
 
 

Performance comparison with APC-Alexa Fluor® 750
 
Human PBMCs were stained with anti-CD3 clone UCHT1 conjugated to
APC-eFluor® 780 (left panel) or APC-Alexa Fluor® 750 (middle panel).
The right panel shows the overlay of both histograms.
 
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  Organic Dyes Products
 
Comparison with Competitor Product
 
  APC-eFluorŽ 780 vs APC-H7  
Comparison with APC-H7
Mouse splenocytes were stained with anti-CD8 (clone 53-6.7) conjugated to APC-eFluor® 780 (blue-solid line) or APC-H7 (red-dotted line).
 
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  Organic Dyes Products
 
APC-eFluor® 780 Emission Spectra
 
  APC-eFluorŽ 780 Emission Spectra
 
 

APC-eFluor® 780 Organic Dye
 
Emission characteristics of an APC-eFluor® 780 conjugated antibody with
the recommended band pass filter (780/60) for this fluorochrome.
 
A 740-nm long pass filter is recommended for use with this fluorochrome.
 
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  Organic Dyes Products
    eFluor® Introduction
  eFluor® Nanocrystals
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