C57BL/6 splenocytes were first stained with Anti-Mouse CD3e FITC (cat. 11-0031), followed by 0.06 ĩg of Anti-Fluorescein isothiocyanate (FITC) Alexa FluorŽ 647. Total viable cells were used for analysis.
Contents: Anti-Fluorescein isothiocyanate (FITC) Alexa FluorŽ 647 Catalog Number: 51-3300 Concentration: 0.2 mg/ml Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: FITC-9 Host/Isotype: Mouse IgG1
Product Options
Cat. No.
Size
Price
Add Qty to Cart
51-3300-80
25 ug
51-3300-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŽ 647 conjugated products with eFluorŽ 660 conjugated products.
Description
This FITC-9 monoclonal antibody reacts to fluorescein isothyocianate (FITC), a derivative of fluorescein commonly used in flow cytometry and fluorescent microscopy. FITC-9 can be used for the separation of cells labeled with FITC-conjugated antibodies or for staining.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This FITC-9 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This FITC-9 antibody has been tested by flow cytometric analysis of cells stained with FITC-conjugated antibody. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 µl. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Butcher EC, Weissman IL. Direct fluorescent labeling of cells with fluorescein or rhodamine isothiocyanate. I. Technical aspects. J Immunol Methods. 1980;37(2):97-108.
The TH, Feltkamp TE. Conjugation of fluorescein isothiocyanate to antibodies. II. A reproducible method. Immunology. 1970 Jun;18(6):875-81.
Hebert GA, Pittman B, Cherry WB. Factors affecting the degree of nonspecific staining given by fluorescein isothiocyanate labelled globulins. J Immunol. 1967 Jun;98(6):1204-12.
Legal
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.