Staining of C57BL/6 splenocytes with Anti-Mouse CD4 Biotin (cat. 13-0041) followed by 0.06 µg of Streptavidin APC-Cy5.5 (blue histogram) or 0.06 µg of Streptavidin eFluor® 710 (pink histogram). Total viable cells were used for analysis.
Contents: Streptavidin eFluor® 710 Catalog Number: 49-4317 Concentration: 0.2 mg/ml Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material.
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Cat. No.
Size
Price
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49-4317-80
25 ug
49-4317-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.
Applications Reported
For research use only, not for diagnostic or therapeutic use. eFluor® 710 Streptavidin has been reported for use in flow cytometric analysis.
The eFluor® 710 fluorochrome has a peak emission of 710 nm, and can be used as a replacement for APC-Cy5.5 and Alexa Fluor 700.
Applications Tested
eFluor® 710 Streptavidin has been tested by flow cytometric analysis of biotin-stained cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.