Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgG1 κ Isotype Control Biotin (cat. 13-4714) (open histogram) or 0.25 µg of Anti-Human IgM Biotin (filled histogram) followed by Streptavidin PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.
Contents: Anti-Human IgM Biotin Catalog Number: 13-9998 Concentration: 0.5 mg/ml Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Clone: SA-DA4 Host/Isotype: Mouse IgG1, κ
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Cat. No.
Size
Price
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13-9998-80
25 ug
13-9998-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The SA-DA4 monoclonal antibody reacts with the μ heavy chain of human IgM. It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The SA-DA4 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The SA-DA4 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.