Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC (cat. 11-0452) and 0.25 µg of Rat IgG2a Isotype Control Biotin (cat. 13-4321) (left) or 0.25 µg of Anti-Mouse IgM Biotin (right) followed by Streptavidin PE (cat. 12-4317). Total viable cells were used for analysis.
Contents: Anti-Mouse IgM Biotin Catalog Number: 13-5890 Concentration: 0.5 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.1% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Clone: eB121-15F9 Host/Isotype: Rat IgG2a, κ
Product Options
Cat. No.
Size
Price
Add Qty to Cart
13-5890-81
50 ug
13-5890-82
100 ug
13-5890-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The eB121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated eB121-15F9 can be used as a detection secondary for mouse IgM.
Applications Reported
For research use only, not for diagnostic or therapeutic use. eB121-15F9 has been reported for use in flow cytometric analysis to detect the surface IgM on mouse B cells and as a second step to detect primary mouse IgMs. It can also be used in ELISA.
Applications Tested
The eB121-15F9 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.