Staining of the IGEL cell line with Anti-Mouse IgE PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Contents: Anti-Mouse IgE FITC Catalog Number: 11-5992 Concentration: 0.5 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: 23G3 Host/Isotype: Rat IgG1, κ
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11-5992-81
50 ug
11-5992-82
100 ug
11-5992-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The 23G3 monoclonal antibody reacts with the ε heavy chain of mouse IgE. It does not react with other classes of mouse immunoglobulin including IgD, IgG, IgA, or IgM.
Applications Reported
For research use only, not for diagnostic or therapeutic use. 23G3 has been reported for use in ELISA and flow cytometric analysis.
Applications Tested
This 23G3 antibody has been tested by ELISA and can be used at 1:5000-1:10000. This 23G3 antibody has also been tested by flow cytometric analysis of IGEL cells, and can be used at less than or equal to 1 µg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Keegan AD, Fratazzi C, Shopes B, Baird B, Conrad DH. 1991. Characterization of new rat anti-mouse IgE monoclonals and their use along with chimeric IgE to further define the site that interacts with Fc epsilon RII and Fc epsilon RI. Mol Immunol. 28(10):1149-54.