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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq. DescriptionThe 364/3B3-14 antibody reacts with human interleukin-1alpha (IL-1α). Applications ReportedFor research use only, not for diagnostic or therapeutic use. The 364/3B3-14 antibody has been reported for use as capture antibody in a human IL-1α ELISA and for intracellular staining for flow cytometric analysis. Applications TestedThe 364/3B3-14 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-1α in combination with the biotinylated 28.9 (13-7115) antibody for detection and recombinant human IL-1α (14-8019) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate. The fluorochrome-conjugated 364/3B3-14 antibody is offered in 2 formats: - µg size: has been tested by intracellular staining for flow cytometric analysis and can be used at less than or equal to 0.5 μg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining for flow cytometric analysis and can be used at 20 μl per million cells in a 100 μl total staining volume. References
Thorpe, R., et al. 1988. Sensitive and specific immunoradiometric assay for human IL-1α. Lymphokine Res. 2: 119-127. Sassi, A., et al. 2005. Mechanisms of the natural reactivity of lymphocytes from noninfected individuals to membrane-associated leishmania infantum antigens. 174: 3598-3607. (ELISA) Caricchio, R., et al. 2003. Ultraviolet B radiation-induced cell death: critical role of ultraviolet dose in inflammation and lupus autoantigen redistribution. J. Immunol. 171: 5778-5786. (Intracellular staining). Garth, L.J., et al. 2001. Dendritic cell activation and cytokine production induced by group B neisseria meningitidis: interleukin-12 production depends on lipopolysaccharide expression in intact bacteria. Infect. Immunity. 69: 4351-4357. (Intracellular staining). Related ProductsCat. 13-7017 Biotin anti-human IL-1a (Interleukin-1 alpha, IL1a) (clone CRM6) Cat. 11-7019 Fluorescein (FITC) anti-human IL-1a (Interleukin-1 alpha, IL1a) (clone CRM8) Cat. 14-7019 Affinity Purified anti-human IL-1a (Interleukin-1 alpha, IL1a) (clone CRM8) Cat. 16-7019 Functional Grade Purified anti-human IL-1a (Interleukin-1 alpha, IL1a) (clone CRM8) Cat. 13-7115 Biotin anti-human IL-1a (Interleukin-1 alpha, IL1a) (clone 28.9) Cat. 16-7115 Functional Grade Purified anti-human IL-1a (Interleukin-1 alpha, IL1a) (clone 28.9) Cat. 00-8222 eBioscience IC Fixation Buffer Cat. 00-8333 eBioscience Permeabilization Buffer(10X) Cat. 88-8823 eBioscience Fixation & Permeabilization |
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