Staining of mouse TRAIL transfected cells with Anti-Mouse CD253 (TRAIL) PE. Autofluorescence is shown via open histogram. Total viable cells were used for analysis.
Contents: Anti-Mouse CD253 (TRAIL) Functional Grade Purified Catalog Number: 16-5951 Concentration: 1 mg/ml Formulation: aqueous buffer, no sodium azide Storage Conditions: Store at 2-8°C. Handling Conditions: Use in sterile envrioment. Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay. Clone: N2B2 Host/Isotype: Rat IgG2a, κ
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16-5951-81
50 ug
16-5951-82
100 ug
16-5951-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The N2B2 monoclonal antibody reacts with mouse TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily. TRAIL is not detected on the surface of freshly isolated T, B, or NK cells, but can be induced preferentially on CD3- NK1.1+ NK cells after stimulation with IL-2 or IL-15. N2B2 inhibits IL-2- or IL-15-activated NK cell cytotoxicity against mouse fibrosarcoma L929 target cells.
Applications Reported
N2B2 has been reported for use in flow cytometric analysis, and blocking of TRAIL in functional assays.
Applications Tested
The N2B2 antibody has been tested by flow cytometric analysis of mouse TRAIL transfected cells and can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Kayagaki, N., N. Yamaguchi, et al. (1999). Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells. J Immunol 163(4): 1906-13.
Hayakawa Y, Screpanti V, Yagita H, Grandien A, Ljunggren HG, Smyth MJ, Chambers BJ.
NK cell TRAIL eliminates immature dendritic cells in vivo and limits dendritic cell vaccination efficacy.J Immunol. 2004 Jan 1;172(1):123-9. (PubMed FA in vivo)
Taieb J, Chaput N, et al. A novel dendritic cell subset involved in tumor immunosurveillance.
Nat Med. 2006 Feb;12(2):214-9. (PubMed FA in vivo)