Staining of non-transfected (left) and mouse DR5-transfected (right) BHK cells with 0.125 µg of Armenian Hamster IgG Isotype Control Functional Grade Purified (cat. 16-4888) (open histogram) or 0.125 µg purified Anti-Mouse CD262 (DR5) Purified (filled histogram) followed by Anti-Armenian Hamster IgG Biotin (cat. 13-4113) and Streptavidin PE (cat. 12-4317). Total viable cells were used for analysis.
Contents: Anti-Mouse CD262 (DR5) Purified Catalog Number: 14-5883 Concentration: 0.5 mg/ml Formulation: aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer Storage Conditions: Store at 2-8°C. Clone: MD5-1 Host/Isotype: Armenian Hamster IgG
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Cat. No.
Size
Price
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14-5883-81
50 ug
14-5883-82
100 ug
14-5883-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The MD5-1 monoclonal antibody reacts with mouse DR5, also known as TRAIL-R2, Apo2, TRICK2, and KILLER. DR5 binds to TRAIL, activates NF-κB, and induces TRAIL-mediated apoptosis. DR5 mRNA is expressed broadly by normal tissue and human antigen is also expressed by some tumor cell lines.
Applications Reported
The MD5-1 antibody has been reported for use in flow cytometric analysis. It has also been reported for use in functional assays. (Please use Functional Grade purified MD5-1, cat. 16-5883, in functional assays.)
Applications Tested
The MD5-1 antibody has been tested by flow cytometric analysis of mouse DR5-transfected cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Sheridan, J. P., S. A. Marster, R. M. Pitti, A. Gurney, M. Skubatch, D. Baldwin, L. Ramakrishnan, C. L. Gray, K. Baker, W. I. Wood, A. D. Goddard, P. Godowski, A. Ashkenazi. 1997. Control of TRAIL-Induced Apoptosis by a Family of Signaling and Decoy Receptors. Science 277:818-821. Takeda K, Yamaguchi N, Akiba H, Kojima Y, Hayakawa Y, Tanner JE, Sayers TJ, Seki N, Okumura K, Yagita H, Smyth MJ. 2004. Induction of Tumor-specific T Cell Immunity by Anti-DR5 Antibody Therapy. J Exp Med. Feb 16;199(4):437-48. Epub 2004 Feb 09.