Staining of the PDV cell line with 0.25 µg of Armenian Hamster IgG Isotype Control Purified (cat. 14-4888) (open histogram) or 0.25 µg of Anti-Mouse Plexin-B2 Purified (filled histogram) followed by Anti-Armenian Hamster IgG Biotin (cat. 13-4113) and Streptavidin PE (cat. 12-4317). Total viable cells were used for analysis.
Contents: Anti-Mouse Plexin-B2 Purified Catalog Number: 14-5665 Concentration: 0.5 mg/ml Formulation: aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer Storage Conditions: Store at 2-8°C. Clone: eBio3E7 (3E7) Host/Isotype: Armenian Hamster IgG
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Size
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14-5665-81
50 ug
14-5665-82
100 ug
14-5665-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
Plexin-B2 is a member of the Semaphorin receptor family. The semaphorin family consists of membrane and secreted proteins involved in attraction and repulsion of neuronal guidance cues. Plexin-B1 is a transmembrane protein that can be cleaved coverting the single chain presursor into a heterodimeric receptor. It has been shown to bind Sema4D/CD100 and is involved in axon development in mouse embryos. Sema4D also plays a role in the immune system interacting with CD72. Plexin-B2's possible role in the immune system has not been characterized.
Applications Reported
This eBio3E7 (3E7) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining.
Applications Tested
This eBio3E7 (3E7) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Artigiani S, Barberis D, Fazzari P, Longati P, Angelini P, van de Loo JW, Comoglio PM, Tamagnone L. Functional regulation of semaphorin receptors by proprotein convertases. J Biol Chem. 2003 Mar 21;278(12):10094-101. (PubMed)
Raper JA. Semaphorins and their receptors in vertebrates and invertebrates. Curr Opin Neurobiol. 2000 Feb;10(1):88-94. (PubMed)
Worzfeld T, Puschel AW, Offermanns S, Kuner R. Plexin-B family members demonstrate non-redundant expression patterns in the developing mouse nervous system: an anatomical basis for morphogenetic effects of Sema4D during development. Eur J Neurosci. 2004 May;19(10):2622-32. (PubMed)