Staining of normal human peripheral blood cells with Anti-Human CD3 APC (cat. 17-0038) and Mouse IgG1 κ Isotype Control FITC (cat. 11-4714) (left) or Anti-Human CD43 FITC (right). Cells in the lymphocyte gate were used for analysis.
Contents: Anti-Human CD43 FITC Catalog Number: 11-0439 Concentration: Suffix -71/73, 20 μL (0.25 μg)/test; Suffix -41/42, 5 μL (0.25 μg)/test Formulation: aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: eBio84-3C1 (84-3C1) Host/Isotype: Mouse IgG1
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Size
Price
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11-0439-71
25 tests
11-0439-73
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŪ 647 conjugated products with eFluorŪ 660 conjugated products.
Description
The monoclonal antibody ebio84-3C1 reacts with CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin. CD43 is a sialomucin which like many mucins can have both adhesive and anti-adhesive functions. Expression of CD43 is found on most leukocytes except resting B lymphocytes. Proteolytic processing upon activation decreases surface expression. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages.
Applications Reported
This eBio84-3C1 (84-3C1) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBio84-3C1 (84-3C1) antibody has been pre-titrated and tested by flow cytometric analysis of peripheral blood mononuclear cells. Refer to catalog number suffix on the vial for amount to use per test: 71/73 are 20 μL (0.25 μg) per test; whereas 41/42 are 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Borche L, Lozano F, Vilella R, Vives J. CD43 monoclonal antibodies recognize the large sialoglycoprotein of human leukocytes. Eur J Immunol. 1987 Oct;17(10):1523-6.
Schlossman, S., L. Bloumsell, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.