Anti-Human CD56 (NCAM) Biotin (See Replacement Clone: CMSSB cat 13-0567)
Also known as: NCAM, Neural cell adhesion molecule
RUO: For Research Use Only
Normal human peripheral blood cells were stain with 1.0 µg of Mouse IgG1 κ Isotype Control Biotin (cat. 13-4714) (purple line) or 1.0 µg of Anti-Human CD56 (NCAM) Biotin (blue line) followed by Streptavidin PE (cat. 12-4317).
Contents: Anti-Human CD56 (NCAM) Biotin (See Replacement Clone: CMSSB cat 13-0567) Catalog Number: 13-0568 Concentration: 0.5 mg/ml Formulation: aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer Storage Conditions: Store at 2-8°C. Do not freeze. Clone: CB56 Host/Isotype: Mouse IgG1
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Cat. No.
Size
Price
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13-0568-80
25 ug
13-0568-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The CB56 monoclonal antibody reacts with human CD56, also known as Neural cell Adhesion Molecule (NCAM). CD56 is a highly glycosylated transmembrane molecule expressed by neurons and plays a role in the homotypic adhesion of neural cells. In the hematopoietic system, CD56 is expressed on NK cells and NKT cells.
Applications Reported
This CB56 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This CB56 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Hayakawa Y, Huntington ND, Nutt SL, Smyth MJ. Functional subsets of mouse natural killer cells. Immunol Rev. 2006 Dec;214:47-55.
Kishimoto, T., A.E.G., von dem Borne, et al. eds. 1998. Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing, Inc. London.