Also known as: Ly49C/I/F/H, Ly-49I, Ly-49F, Ly-49H, NK cell subset
RUO: For Research Use Only
Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin α2) FITC (cat. 11-5971) and staining buffer (autofluorescence) (left) or 0.125 µg of Anti-Mouse Ly-49C/I/F/H PE (right). Total viable cells were used for analysis.
Contents: Anti-Mouse Ly-49C/I/F/H PE Catalog Number: 12-5991 Concentration: 0.2 mg/ml Formulation: aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: 14B11 Host/Isotype: Golden Syrian Hamster IgG
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12-5991-63
10 ug
12-5991-81
50 ug
12-5991-82
100 ug
12-5991-83
200 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The 14B11 monoclonal antibody reacts with a subset of mouse Ly-49 receptors and recognizes a majority of mouse NK cells. This antibody reacts with 3 putative inhibitory members of the Ly-49 family, F, I, and C and one putative activating receptor, Ly-49H. 14B11 does not react with Ly-49A, B, D, or G. It is reported that 14B11 enhances the redirected lysis of FcR+ target cells by IL-2-activated NK cells.
Applications Reported
The 14B11 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The 14B11 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Coles, M. C., C. W. McMahon, et al. (2000). "Memory CD8 T lymphocytes express inhibitory MHC-specific Ly49 receptors." Eur J Immunol 30(1): 236-44. Corral, L., H. Takizawa, et al. (1999). "A new monoclonal antibody reactive with several Ly49 NK cell receptors mediates redirected lysis of target cells." Hybridoma 18(4): 359-66.