human TLR4 (CD284, Toll-like receptor 4, TLR-4)

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Contents: Alexa Fluor® 700 anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)
Catalog Number: 56-9917
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, gelatin and 0.2% BSA for stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HTA125
Isotype: Mouse IgG2a, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
56-9917-71	25 tests	$180
56-9917-73	100 tests	$350

*International customers: Please contact your distributor for region specific pricing.

Staining of human TLR4-transfected cells with staining buffer (autofluorescence) (open histogram) or Alexa Fluor® 700 anti-human TLR4 (HTA125) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-9917	PE anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)	488	575	FC
13-9917	Biotin anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)	N/A	N/A	FC
14-9917	Affinity Purified anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)	N/A	N/A	FA FC IHC(Paraffin) IP
16-9917	Functional Grade* Purified anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)	N/A	N/A	FA FC
17-9917	APC anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)	633	660	FC
25-9917	Phycoerythrin-Cy7 (PE-Cy7) anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)	488	760	FC
51-9917	Alexa Fluor® 647 anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)	633	668	FC
53-9917	Alexa Fluor® 488 anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)	488	519	FC
56-9917	Alexa Fluor® 700 anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4) (APC-Cy5.5 Equivalent)	633	723	FC
57-9917	Pacific Blue® anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HTA125 monoclonal antibody reacts with human Toll-like receptor 4 (TLR4). So far, at least ten members of the Toll family have been identified in humans. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with another molecule called MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. TLR4 is expressed by peripheral blood monocytes. HTA125 has been reported to immunoprecipitate human TLR4 (~100 kDa) from transfected cells. Most TLR cell surface expression, especially TLR1 and TLR4, occurs at low levels on monocytes and at even lower levels on other cell types including granulocytes and immature dendritic cells (iDC). Furthermore, a relatively high degree of variability in TLR surface expression has been reported among normal donors. For more information about detection of TLRs by flow cytometric analysis, please visit our website at http://www.ebioscience.com/ebioscience/appls/TLR_FC.htm.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HTA125 antibody has been reported for use in flow cytometric analysis.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This HTA125 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (1.0 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Tabeta, K., K. Yamazaki, et al. (2000). "Toll-like receptors confer responsiveness to lipopolysaccharide from Porphyromonas gingivalis in human gingival fibroblasts." Infect Immun 68(6): 3731-5.
Akashi, S., H. Ogata, et al. (2000). "Regulatory roles for CD14 and phosphatidylinositol in the signaling via toll-like receptor 4-MD-2." Biochem Biophys Res Commun 268(1): 172-7.
Shimazu, R., S. Akashi, et al. (1999). "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." J Exp Med 189(11): 1777-82.

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Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.