Also known as: MCP1, Monocyte chemotactic protein 1, CCL-2
RUO: For Research Use Only
Contents: Mouse CCL2 (MCP-1) ELISA Ready-SET-Go!® Catalog Number: 88-7391 Sensitivity: 15 pg/ml Standard Curve Range: 15 - 2000 pg/ml Storage Conditions: Store at 2-8°C except standard which should be stored at less than or equal to -70°C.
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Size
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88-7391-22
2 plates (includes plates)
88-7391-76
20 plates (includes plates)
88-7391-77
20 plates
88-7391-86
10 plates (includes plates)
88-7391-88
10 plates
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
This Mouse MCP-1 (CCL2) ELISA Ready-SET-Go! reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This ELISA reagent set is specifically engineered for accurate and precise measurement of mouse MCP-1 protein levels from samples including serum, plasma, and supernatants from cell cultures.
Components
Capture Antibody. Pre-titrated, purified antibody Detection Antibody. Pre-titrated, biotin-conjugated antibody Standard. Recombinant cytokine for generating standard curve and calibrating samples ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20). Assay Diluent. 5X concentrated Detection enzyme. Pre-titrated Avidin-HRP Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards 96 Well Plate. Corning Costar 9018 (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)
Applications Reported
For research use only, not for diagnostic or therapeutic use.
References
Soehnlein O, Zernecke A, Eriksson EE, Rothfuchs AG, Pham CT, Herwald H, Bidzhekov K, Rottenberg ME, Weber C, Lindbom L. Neutrophil secretion products pave the way for inflammatory monocytes. Blood. 2008 Aug 15;112(4):1461-71. (RSG ELISA kit, lavage, PubMed)
Senaratne RH, Sidders B, Sequeira P, Saunders G, Dunphy K, Marjanovic O, Reader JR, Lima P, Chan S, Kendall S, McFadden J, Riley LW. Mycobacterium tuberculosis strains disrupted in mce3 and mce4 operons are attenuated in mice. J Med Microbiol. 2008 Feb;57(Pt 2):164-70. (RSG ELISA kit, TC supernatant, PubMed)
Protocol
To view protocol, please download the TDS by clicking the PDF button above.