Staining of 1-day LPS-stimulated human peripheral blood cells with 0.5 µg of Armenian Hamster IgG Isotype Control FITC (cat. 11-4888) (open histogram) or 0.5 µg of Anti-CCL2 (MCP-1) FITC (filled histogram). Cells in the monocyte gate were used for analysis.
Contents: Anti-CCL2 (MCP-1) FITC Catalog Number: 11-7096 Concentration: μg size: 0.5 mg/ml; test size: 5 μl (0.5 µg)/test Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: 2H5 Host/Isotype: Armenian Hamster IgG
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Cat. No.
Size
Price
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11-7096-41
25 tests
11-7096-81
50 ug
11-7096-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The 2H5 monoclonal antibody reacts with mouse, rat, and human monocyte chemoattractant protein-1 (MCP-1), also known as CCL2 and MCAF.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 2H5 antibody has been reported for use in ELISA, intracellular staining for flow cytometric analysis, and cytokine neutralization. (Please use Functional Grade purified 2H5 antibody, cat. 16-7096, in functional assays.)
Applications Tested
This 2H5 antibody is offered in 2 formats:
- μg size: has been tested by intracellular staining and flow cytometric analysis of thioglycolate-induced peritoneal exudate cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
- test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of LPS-stimulated normal human peripheral blood mononuclear cells. This can be used at test size: 5 μl (0.5 μg)/test per test. The pre-titrated test size contains 0.2% BSA.
References
Luo, Y., et al. 1994. J Immunol. 153: 3708. [ Antibody generation, ELISA, immunoblotting, in vitro neutralization]
Morrison, B., et al. 2003. J. Clin. Invest. 112: 1862. [In vivo neutralization]
Taal MW, Chertow GM, et al. 2001. Mechanisms underlying renoprotection during renin-angiotensin system blockade. Am J Physiol Renal Physiol. 280(2):F343-55. (2H5, IHC frozen in rat, PubMed)
Luo Y, Fischer FR, et al. 2000. Macrophage inflammatory protein-2 and KC induce chemokine production by mouse astrocytes. J Immunol. 165(7):4015-23. (2H5, IHC frozen, PubMed)