Staining of unstimulated (left) or 3-day LPS-stimulated (right) C57BL/6 splenocytes with 0.03 µg of Rat IgG2a κ Isotype Control APC (cat. 17-4321) (open histogram) or 0.03 µg of Anti-Mouse CD86 (B7-2) APC (filled histogram). Total viable cells were used for analysis.
Contents: Anti-Mouse CD86 (B7-2) APC Catalog Number: 17-0862 Concentration: 0.2 mg/mL Formulation: aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: GL1 Host/Isotype: Rat IgG2a, κ
Product Options
Cat. No.
Size
Price
Add Qty to Cart
17-0862-81
50 ug
17-0862-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The GL1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 & CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). This interaction plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation, supports its major contribution during the primary phase of an immune response.
Applications Reported
The GL1 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The GL1 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocyte suspensions. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Hathcock, K. S., G. Laszlo, et al. (1993). "Identification of an alternative CTLA-4 ligand costimulatory for T cell activation [see comments]." Science 262(5135): 905-7. Freeman, G. J., F. Borriello, et al. (1993). "Murine B7-2, an alternative CTLA4 counter-receptor that costimulates T cell proliferation and interleukin 2 production." J Exp Med 178(6): 2185-92. Inaba, K., M. Witmer-Pack, et al. (1994). "The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro." J Exp Med 180(5): 1849-60. Hathcock, K. S., G. Laszlo, et al. (1994). "Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function." J Exp Med 180(2): 631-40