Anti-human CD36 Alexa 647 (Refer to NEW eFluorŽ 660 format, cat. 50-0369)
Also known as: GR4, GP3b, GP IIIb, Thrombospondin Receptor
RUO: For Research Use Only
Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (blue histogram) or Anti-Human CD36 Alexa FluorŽ 647 (purple histogram). Cells in the monocyte gate were used for analysis.
Contents: Anti-human CD36 Alexa 647 (Refer to NEW eFluorŽ 660 format, cat. 50-0369) Catalog Number: 51-0369 Concentration: 5 μl (0.125 μg)/test Formulation: aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: eBioNL07 (NL07) Host/Isotype: Mouse IgM
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51-0369-41
25 tests
51-0369-42
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŽ 647 conjugated products with eFluorŽ 660 conjugated products.
Description
The monoclonal antibody eBioNL07 recognizes human CD36, which is a member of the class B scavenger receptor family. CD36 was originally identified as a platelet-membrane glycoprotein also called glycoprotein IV and a receptor for thrombospondin-1 (TSP-1) and extracellular matrix proteins. Binding to TSP-1 is in the CLESH (CD36 LIMP-II Emp sequence homology) domain of CD36. CD36 expression is broad and includes microvascular (but not large vessel) endothelium, adipocytes, skeletal muscle, dendritic cells, epithelia of the retina, breast, and intestine, smooth muscle cells, and hematopoietic cells, including erythroid precursors, platelets, monocytes/macrophages, DCs and megakaryocytes. Expression on platelets is absent on Naka negative donors. Unlike other scavenger receptor, CD36 binds LDL that has been exposed to minimally oxidizing conditions. CD36 is also a fatty acid translocase (FAT) necessary for the transport of long-chain fatty acids (LCFAs) and therefore may play a role in atherosclerosis.
Applications Reported
This eBioNL07 (NL07) antibody has been reported for use in flow cytometric analysis.
NOTE: eBioscience is in the process of replacing Alexa FluorŽ 647-conjugated antibodies with eFluorŽ 660-conjugated antibodies. This Alexa FluorŽ 647 product will only be available for a limited time. For more information on the replacement of our Alexa FluorŽ 647 products with eFluorŽ 660, click here.
Applications Tested
This eBioNL07 (NL07) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μl (0.125 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 ĩL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Alessio M, Greco NJ, Primo L, Ghigo D, Bosia A, Tandon NN, Ockenhouse CF, Jamieson GA, Malavasi F. Platelet activation and inhibition of malarial cytoadherence by the anti-CD36 IgM monoclonal antibody NL07.
Blood. 1993 Dec 15;82(12):3637-47. (NL07, FC, PubMed)
Alessio M, Ghigo D, Garbarino G, Geuna M, Malavasi F. Analysis of the human CD36 leucocyte differentiation antigen by means of the monoclonal antibody NL07.
Cell Immunol. 1991 Oct 15;137(2):487-500. (NL07, FC, PubMed)
Gruarin P, Ulliers D, Thorne RF, Alessio M. Methionine 156 in the immunodominant domain of CD36 contributes to define the epitope recognized by the NL07 MoAb.
Mol Cell Biochem. 2000 Nov;214(1-2):89-95. (NL07, PubMed)
Legal
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.