human CCR6/CD196 antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-human CD196 (CCR6)
Catalog Number: 14-1969
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: R6H1
Isotype: Mouse IgG1

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14-1969-80

25 ug

14-1969-82

100 ug

Immunoblotting of 10⁶ normal peripheral blood cells with 5 μg/mL anti-human CD196 antibody. The band was visualized using HRP-conjugated rat anti-mouse IgG.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1969	Affinity Purified anti-human CD196 (CCR6)	N/A	N/A	FC IP WB
11-1969	Fluorescein isothiocyanate (FITC) anti-human CD196 (CCR6)	488	518	FC
12-1969	Phycoerythrin (PE) anti-human CD196 (CCR6)	488	575	FC
25-1969	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD196 (CCR6)	488	760	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

This R6H1 monoclonal antibody reacts with CD196 (also known as CCR6), a seven transmembrane G protein-coupled receptor expressed on T, B, dendritic, natural killer, and Langerhans cells. This CC chemokine receptor uniquely binds MIP-3a/CCL20. A chemoattractant for dendritic cells, effector/memory T cells, and B cells, CD196 is also involved in host defense and inflammation at epithelial sites. Furthermore, this receptor has been implicated in Th17 differentiation and CD4+FoxP3+ regulatory T cell development.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This R6H1 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunoprecipitation.

Applications Tested

This R6H1 antibody has been tested by western blot on lysates prepared from normal human peripheral blood cells. This antibody can be used at less than or equal to 5 ug/mL.

References

Liu H, Rohowsky-Kochan C.Regulation of IL-17 in human CCR6+ effector memory T cells. J Immunol. 2008 Jun 15;180(12):7948-57

Singh SP, Zhang HH, Foley JF, Hedrick MN, Farber JM.Human T cells that are able to produce IL-17 express the chemokine receptor CCR6. J Immunol. 2008 Jan 1;180(1):214-21

Lim HW, Lee J, Hillsamer P, Kim CH.Human Th17 cells share major trafficking receptors with both polarized effector T cells and FOXP3+ regulatory T cells. J Immunol. 2008 Jan 1;180(1):122-9

Shutyser E, Struyf S, Van Damme J.The CC chemokine CCL20 and its receptor CCR6. Cytokine Growth Factor Rev. 2003 Oct;14(5):409-26.

Carramolino L, Kremer L, Goya I, Varona R, Buesa JM, Gutiérrez J, Zaballos A, Martínez-A C, Márquez G. Down-regulation of the beta-chemokine receptor CCR6 in dendritic cells mediated by TNF-alpha and IL-4. J Leukoc Biol. 1999 Nov;66(5):837-44. (clone R6H1, FC, Pubmed)

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