|
||||||||||||||||||||||
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Description | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
The eBioHMb1-1 monoclonal antibody reacts with mouse and rat CD29 (integrin β1), a 110-120 kDa member of the β integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. CD29 binds non-covalently with the α integrins CD49a-f to form the VLA-1 through VLA-6 complexes, as well as with CD51. These αβ integrin heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. All integrins which include CD29 bind to extracellular matrix proteins including collagen, laminin, fibronectin and vitronectin, whereas some CD29-containing integrins can also interact with cellular receptors such as VCAM-1 and MadCAM-1. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications Reported | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
This eBioHMb1-1 (HMb1-1) antibody has been reported for use in flow cytometric analysis. This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications Tested | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
This eBioHMb1-1 (HMb1-1) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
References | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Noto K, Kato K, Okumura K, Yagita H.
Identification and functional characterization of mouse CD29 with a mAb.
Int Immunol. 1995 May;7(5):835-42. (HMβ1-1, mAb development, IP, FA, PubMed) Ridger VC, Wagner BE, Wallace WA, Hellewell PG. Differential effects of CD18, CD29, and CD49 integrin subunit inhibition on neutrophil migration in pulmonary inflammation. J Immunol. 2001 Mar 1;166(5):3484-90. (HMβ1-1, FA, PubMed) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Legal | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT # 5,268,486, 5,569,587 AND 5,627,027 AND FOREIGN EQUIVALENTS AND PENDING APPLICATIONS. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE. COMMERCIAL USE shall include: 1. sale, lease, license or other transfer of the material or any material derived or produced from it; 2. sale, lease, license or other grant of rights to use this Material or any material derived or produced from it; 2. use of this material to perform services for a fee for third parties. IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE, RETURN THIS MATERIAL, UNOPENED TO EBIOSCIENCE, INC. 10255 SCIENCE CENTER DRIVE, SAN DIEGO, CALIFORNIA 92121 USA AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED. |
Home | Index | Site Map | FAQ | Terms & Conditions | Distributors | Careers | Contact Us |
Copyright © 2000-2010 eBioscience, Inc. Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions. |