Cell lysates prepared from Jurkat cells left untreated (lane 1) or treated for 2 hrs with staurosporine (lane 2) were immunoblotted with 0.1 μg/ml of the Anti-Human PARP1 (Cleaved) antibody. Bands were visualized using Anti-Mouse IgG HRP.
Contents: Anti-Human PARP1 (Cleaved) Purified Catalog Number: 14-6668 Concentration: 0.5 mg/mL Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Clone: HLNC4 Host/Isotype: Mouse IgG1, k
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14-6668-80
25 ug
14-6668-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŪ 647 conjugated products with eFluorŪ 660 conjugated products.
Description
This HLNC4 monoclonal antibody reacts with human poly (ADP-ribose) polymerase (PARP1). This ubiquitous 116 kDa nuclear enzyme is involved in DNA repair. During apoptosis, active caspases -3, -6 and -7 cleave PARP1 after Asp214, thereby inactivating PARP1 and generating two apoptotic fragments sized 85 kDa and 25 kDa.
The HLNC4 antibody specifically recognizes the 85 kDa PARP1 fragment produced after cleavage and does not recognize the full-length 116 kDa protein.The following peptide was used as the immunogen: NH2-GVDEVAKKKSKKEKDC-COOH.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This HLNC4 antibody has been reported for use in immunoblotting (WB).
Applications Tested
This HLNC4 antibody has been tested by immunoblotting of staurosporine-treated Jurkat cells. This can be used at less than or equal to 0.1 μg/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Patel T, Gores GJ, Kaufmann SH. The role of proteases during apoptosis. FASEB J. 1996. Apr;10(5):587-97. Review.
Tewari M, Quan LT, O'Rourke K, Desnoyers S, Zeng Z, Beidler DR, Poirier GG, Salvesen GS, Dixit VM. Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell. 1995. Jun 2;81(5):801-9.
Lamarre D, Talbot B, de Murcia G, Laplante C, Leduc Y, Mazen A, Poirier GG. Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study. Biochim Biophys Acta. 1988. Jul 13;950(2):147-60.
Lamarre D, Talbot B, Leduc Y, Muller S, Poirier G. Production and characterization of monoclonal antibodies specific for the functional domains of poly(ADP-ribose) polymerase. Biochem Cell Biol. 1986. Apr;64(4):368-76.
Zahradka P., Ebisuzaki K. Poly(ADP-ribose) polymerase is a zinc metalloenzyme. Eur. J. Biochem. 1984.142: 503-509.