Fluorescence profiles of normal human peripheral blood lymphocytes unstained (blue histogram) or stained intracellularly with PE anti-human ZAP-70 (purple histogram).
Contents: ZAP 70 (1E7.2) PE Catalog Number: 9012-6695 Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Clone: 1E7.2 Concentration: 5 µl (0.5 µg)/test (a test is defined as the amount that will stain 1x106 cells in 100 µL) Host/Isotype: Mouse IgG1, k Storage Conditions: Store at 2-8°C. Do not freeze. Light-sensitive material.
The 1E7.2 antibody reacts with human and mouse ZAP-70, the TCR?-associated protein-70. ZAP-70 is a cytosolic protein tyrosine kinase (PTK) and a member of the Syk family of proteins. It is expressed in T and NK cells and is required for TCR signaling and development. ZAP-70 interacts with the TCR complex by binding to tyrosine-phosphorylated immunoreceptor tyrosine-based activation motifs (ITAMs) present in the invariant subunits of the TCR complex. Following activation, ZAP-70 is phosphorylated on several tyrosine residues by two mechanisms; an autophosphorylation and a transphosphorylation by the Src family tyrosine kinase Lck1-3. Tyrosine phosphorylation of ZAP-70 correlates to its increased kinase activity and triggers downstream signaling events. Mutations in ZAP-70 have been shown to result in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. 1E7.2 was generated against a KLH-peptide sequence corresponding to the human ZAP-70 amino acid residues 282-307. While ZAP-70 is normally expressed in T and NK cells, several recent studies have also shown high correlation of ZAP-70 positive expression with mutated IgVH expression in B-chronic lymphocytic leukemia (CCL). In conclusion, the expression of ZAP-70, which can be measured by intracellular flow cytometry, may serve as a prognostic marker for B-CLL.
References
Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture (H3-A6), 3rd Edition published by the National Committee for Clinical Laboratory Standards.
Straus DB, Weiss A. The CD3 chains of the T cell antigen receptor associate with the ZAP-70 tyrosine kinase and are tyrosine phosphorylated after receptor stimulation. J Exp Med. 1993;178:1523-1530.
Chan AC, Iwashima M, Turch CW, Weiss A. ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR? chain. Cell. 1992;71:649-662.