Fluorescence profiles of normal human peripheral blood granulocytes unstained (blue histogram) or stained with FITC anti-human CD10 (purple histogram).
Contents: CD10 FITC Catalog Number: 9011-0108 Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Clone: SN5c Concentration: 5 µl (0.5 µg)/test (a test is defined as the amount that will stain 1x106 cells in 100 µL) Host/Isotype: Mouse IgG1 HLDA Workshop: VI Storage Conditions: Store at 2-8°C. Do not freeze. Light-sensitive material.
The SN5c monoclonal antibody recognizes human CD10 (CALLA, NEP, enkephalinase, Neprilysin), which is a 100 kDa, type II cell surface glycoprotein. CD10 was shown to be the same molecule as neutral endopeptidase (NEP), or KII-NA. CD10 is a Zn2+-dependent metallopeptidase acting on substrates such as endothelin, glucagon, gastrin, neurotensin and bradykinin. CD10 is involved in the regulation of chemotactic and inflammatory processes involving neutrophils. In B cells, CD10 regulates stromal cell-dependent B lymphopoiesis and its expression has been reported on mature germinal center B cells. CD10 expression is also found on normal donor granulocytes and bone marrow stromal cells.
References
Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture (H3-A6), 3rd Edition published by the National Committee for Clinical Laboratory Standards.
Kishimoto T, von dem Borne AE, et al, eds. Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London. 1998.