Biotin anti-rat αβ TCR (TCR αβ)

RUO: For Research Use Only

rat alpha beta TCR antibody, Biotin conjugate

Surface staining of rat splenocytes with anti-rat TCR α/β (R73) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Contents: Biotin anti-rat αβ TCR (TCR αβ)
Catalog Number: 13-5960
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Clone: R73
Host/Isotype: Mouse IgG1
Storage Conditions: Store at 4°C.
DO NOT FREEZE.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
13-5960-81	50 ug	To view relevant pricing, please select your country and resume browsing.
13-5960-85	500 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
11-4317	Streptavidin-FITC (Fluorescein isothiocyanate)
12-4317	Streptavidin-PE (Phycoerythrin)
13-4714	Biotin Mouse IgG1, K Isotype Control
17-4317	Streptavidin Allophycocyanin (SA-APC)

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
11-5960	Fluorescein (FITC) anti-rat alpha beta TCR (abTCR, TCRab)	488	518	FC	RUO
12-5960	Phycoerythrin (PE) anti-rat alpha beta TCR (abTCR, TCRab)	488	575	FC	RUO
13-5960	Biotin anti-rat alpha beta TCR (abTCR, TCRab)	N/A	N/A	FC	RUO
14-5960	Affinity Purified anti-rat alpha beta TCR (abTCR, TCRab)	N/A	N/A	FA FC IHC IP WB	RUO
16-5960	Functional Grade* Purified anti-rat alpha beta TCR (abTCR, TCRab)	N/A	N/A	FA FC	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Description

The R73 monoclonal antibody reacts with the α/β chain of rat TCR. αβ TCR is expressed by a majority of thymocytes and peripheral T cells, and intestinal intraepithelial lymphocytes. Cross-linking of the TCR complex with R73 induces the differentiation and activation of T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The R73 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The R73 antibody has been tested by flow cytometric analysis. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Hunig, T., H.J. Wallny, J.K. Hartley, A. Lawetzky, G. Tiefenthaler. (1989). "A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes." J Exp Med. 169: 73-86.
Pieters, R.H.H., P. Punt, M. Bol, J.M. van Dijken, W. Seinen, A.H. Penninks. (1995). "The thymus atrophy inducing organotin compound DBTC stimulates TCRab–CD3 signaling in immature rat thymocytes." Biochem Biophys Res Commun. 214: 552-58.
Fangman, J., R. Schwinzer, K. Wonigeit. (1991). "Unusual phenotype of intestinal intraepithelial lymphocytes in the rat: predominance of T cell receptor a/b+/CD2-cells and high expression of the RT6 alloantigen." Eur J Immunol. 21:753-60.
Brenan, M., D.J.G. Rees. (1997). "Sequence analysis of rat integrin aE1 and aE2 subunits: tissue expression reveals phenotypic similarities between intraepithelial lymphocytes and dendritic cells in lymph." Eur J Immunol. 27: 3070-79.