Staining of Lou rat splenocytes with 0.25 µg of Mouse IgG1 κ Isotype Control Purified (cat. 14-4714) (blue histogram) or 0.25 µg of Anti-Rat CD8b Functional Grade Purified (purple histogram) followed by F(ab')2 Anti-Mouse IgG PE (cat. 12-4012). Total viable cells were used for analysis.
Contents: Anti-Rat CD8b Functional Grade Purified Catalog Number: 16-0080 Concentration: 1 mg/ml Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 2-8°C. Handling Conditions: Use in sterile envrioment. Clone: eBio341 (341) Host/Isotype: Mouse IgG1, κ
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Cat. No.
Size
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16-0080-81
50 ug
16-0080-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The eBio341 monoclonal antibody reacts with rat CD8β. CD8β is a member of the immunoglobulin superfamily which heterodimerizes with CD8α to form the CD8 antigen expressed on CD4+CD8+, double-positive thymocytes, the majority of MHC class I-restricted peripheral T cells, and on some macrophages. CD8β expression is absent in athymic mice, which are populated by T-cells expressing CD8α homodimers. This has led to the idea that CD8β expression is dependent on the development of T cells in the thymus. CD8 binds to MHC class I expressed on the surface of antigen-presenting cells during antigen presentation, and participates in T-cell receptor signal transduction through association with the kinase Lck.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio341 (341) antibody has been reported for use in flow cytometric analysis and functional assays.
Applications Tested
This eBio341 (341) antibody has been tested by flow cytometric analysis of rat splenocytes and thymocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Torres-Nagel N, Kraus E, Brown MH, Tiefenthaler G, Mitnacht R, Williams AF, Hunig T.
Differential thymus dependence of rat CD8 isoform expression.
Eur J Immunol. 1992 Nov;22(11):2841-8. (341, FC, FA, PubMed)
Kuhnlein P, Park JH, Herrmann T, Elbe A, Hunig T.
Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor.
J Immunol. 1994 Aug 1;153(3):979-86. (341, FC, PubMed)
Hirji N, Lin TJ, Bissonnette E, Belosevic M, Befus AD.
Mechanisms of macrophage stimulation through CD8: macrophage CD8alpha and CD8beta induce nitric oxide production and associated killing of the parasite Leishmania major.
J Immunol. 1998 Jun 15;160(12):6004-11. (341, FA, PubMed)