Surface staining of LPS stimulated rat splenocytes with Anti-Rat CD80 (B7-1) PE. Autofluorescence is indicated by open histogram. Total viable cells were used for analysis.
Contents: Anti-Rat CD80 (B7-1) Functional Grade Purified Catalog Number: 16-0800 Concentration: 1 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, No NaN3 Storage Conditions: Store at 2-8°C. Handling Conditions: Use in sterile envrioment. Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay. Clone: 3H5 Host/Isotype: Mouse IgG1, κ
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16-0800-81
50 ug
16-0800-82
100 ug
16-0800-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The 3H5 monoclonal antibody reacts with rat CD80 (B7-1), an ~70 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively. 3H5 blocks binding of CD80 to its ligands.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 3H5 antibody has been reported for use in flow cytometric analysis. It has also been reported in functional studies.
Applications Tested
The 3H5 antibody has been tested by flow cytometric analysis of activated rat splenocyte suspensions. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Maeda, K., T. Sato, et al. (1997). "Characterization of rat CD80 and CD86 by molecular cloning and mAb." Int Immunol 9(7): 993-1000. Damoiseaux, J. G., H. Yagita, et al. (1998). "Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells." J Leukoc Biol 64(6): 803-9.