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Description | |||||||||||||||||||||||||
This mouse IL-33 ELISA Ready-SET-Go! reagent set (with or without high affinity-binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This ELISA reagent set is specifically engineered for precise measurement of mouse IL-33 protein levels from samples, including serum, plasma, and cell culture supernatants. Mouse IL-33, also known as NF-HEV and DVS 27, is a 30-kDa member of the IL-1 family produced by endothelial and epithelial cells. IL-33 plays a pro-inflammatory role in the immune system by inducing IL-5 and IL-13 and by activating basophils, eosinophils and mast cells. In addition, IL-33 decreases inflammation by interacting with IL-1 and blocking its effect. Expression of IL-33 can be upregulated by IL-1β. In addition to gastrointestinal and lung tissue, IL-33 is expressed in the central nervous system, specifically astrocytes. This cytokine is localized primarily in the nucleus where it is thought to play a role in transcriptional repression. Upon wounding or infection, IL-33 is released from the cell via an atypical secretory pathway. Necrosis-mediated release of IL-33 enables binding to ST2, a member of the IL-1 receptor family found on mast and Th2 effector cells, leading to the release of Th2 cytokines. During apoptosis, IL-33 is cleaved by caspases-3 and 7 (and most likely not caspase-1) to produce an 18-kDa fragment which is released. This cleaved form of IL-33 can bind the ST2 receptor, but no signaling events are initiated. This dual pattern of expression (nuclear and extracellular) is reminiscent of "alarmins" also known as endogeneous danger signals. Other known alarmins are IL-1a and HMGB1. | |||||||||||||||||||||||||
Components | |||||||||||||||||||||||||
Capture Antibody. Pre-titrated, purified antibody Detection Antibody. Pre-titrated, biotin-conjugated antibody Standard. Recombinant cytokine for generating standard curve and calibrating samples Coating Buffer. 10X PBS ELISA Coating Buffer Assay Diluent. 5X concentrated Detection enzyme. Pre-titrated Avidin-HRP Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards 96 Well Plate. NUNC Maxisorp flat-bottom (included with product Cat. #’s ending in suffixes -22, -44, -76, -86) | |||||||||||||||||||||||||
Applications Reported | |||||||||||||||||||||||||
For research use only, not for diagnostic or therapeutic use. Mouse IL-33 ELISA RSG! has been reported for use in ELISAset. | |||||||||||||||||||||||||
References | |||||||||||||||||||||||||
Lüthi AU, Cullen SP, McNeela EA, Duriez PJ, Afonina IS, Sheridan C, Brumatti G, Taylor RC, Kersse K, Vandenabeele P, Lavelle EC, Martin SJ. Suppression of interleukin-33 bioactivity through proteolysis by apoptotic caspases. Immunity. 2009 Jul 17;31(1):84-98. Verri WA Jr, Guerrero AT, Fukada SY, Valerio DA, Cunha TM, Xu D, Ferreira SH, Liew FY, Cunha FQ. IL-33 mediates antigen-induced cutaneous and articular hypernociception in mice. Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2723-8. Epub 2008 Feb 4. Schmitz J, Owyang A, Oldham E, Song Y, Murphy E, McClanahan TK, Zurawski G, Moshrefi M, Qin J, Li X, Gorman DM, Bazan JF, Kastelein RA. IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity. 2005 Nov;23(5):479-90. | |||||||||||||||||||||||||
Protocol | |||||||||||||||||||||||||
To view protocol, please download the TDS by clicking the PDF button above. |
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