Also known as: Tumor Necrosis Factor alpha, TNFa TNF-a
RUO: For Research Use Only
Contents: Mouse TNF alpha Recombinant Protein Carrier-Free Catalog Number: 34-8321 Formulation: Sterile liquid; 10 mM sodium phosphate, pH 7.2, 150 mM NaCl, with no carriers or preservatives. 0.22 µm filtered. Storage Conditions: Store at less than or equal to -70°C. Handling Conditions: For best recovery, quick-spin vial prior to opening. Use in sterile envrioment. Source: E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF-α (accession # NM_013693). Molecular Mass: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,258. The DTT-reduced and non-reduced protein migrate as 17 kDa polypeptides on SDS-PAGE. Purity: Greater than 98%, as determined by SDS-PAGE Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Bioactivity: Measured by mouse L929 cytotoxicity assay. The ED50 is 5.0 pg/ml, corresponding to a specific activity of 2.0 x 10E8 Units/mg.
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34-8321-63
10 ug
34-8321-82
100 ug
34-8321-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
Mouse TNF-a is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF-α is expressed as a membrane-bound molecule. TNF-α is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF-α have been observed.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Recombinant mouse TNF-α is biologically active and can induce cytolysis of the L929 mouse cell line in culture. It is also useful as standard for mouse TNF-α ELISA.
Applications Tested
The recombinant mouse TNF-α has been tested by sandwich ELISA using the mouse TNF-α ELISA antibody pair (TN3-19.12/polyclonal antibody) and the alternate mouse TNF-α ELISA antibody pair (1F3F3D4/monoclonal antibody cocktail) and in bioassays using the mouse cell line L929. The ED50 measured in a L929 killing assay is typically 5 pg/ml, corresponding to a specific activity of approximately 2.0 x108 Units/mg.