Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The DB-1 monoclonal antibody reacts with rat interferon-gamma (IFN-γ), a 15-20 kDa cytokine secreted by Th1 cells. For optimal detection of rat IFN-γ by sandwich ELISA, a combination of purified DB-1 for capture and biotinylated polyclonal anti-rat IFN-γ for detection is recommended. DB-1 is used for capture of rat IFN-γ by ELISA.
Applications Reported
DB-1 has been reported for use in capture of rat IFN-γ by ELISA.
Applications Tested
The DB-1 (DB1) antibody has been tested as the capture antibody in a sandwich ELISA for analysis of rat IFNg (Interferon-gamma, IFN-g) in combination with the biotin anti-rat IFNg poly (13-7320) antibody for detection and recombinant rat IFNg (14-8315) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.