human IL-10 antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-human interleukin-10 (IL-10)
Catalog Number: 14-7108
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: JES3-9D7
Isotype: Rat IgG1, κ

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14-7108-81

50 ug

14-7108-85

500 ug

Human peripheral blood mononuclear cells were stimulated with PMA and ionomycin in the presence of Brefeldin A for 5 hours. The cells were surface stained with FITC anti-human CD4 (RPA-T4) and intracellularly stained with PE anti-human IL-10 (JES3-9D7).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7108	Affinity Purified anti-human IL-10 (Interleukin-10, IL10)	N/A	N/A	ELISA cap ELISPOT cap IHC(Paraffin)
16-7108	Functional Grade* Purified anti-human IL-10 (Interleukin-10, IL10)	N/A	N/A	ELISPOT cap NU
12-7108	PE anti-human IL-10 (Interleukin-10, IL10)	488	575	IC Flow
51-7108	Alexa Fluor® 647 anti-human IL-10 (Interleukin-10, IL10)	633	668	IC Flow
53-7108	Alexa Fluor® 488 anti-human IL-10 (Interleukin-10, IL10)	488	519	IC Flow
57-7108	Pacific Blue® anti-human IL-10 (Interleukin-10, IL10)	402	421	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).

Applications Reported

For research use only, not for diagnostic or therapeutic use. The JES3-9D7 antibody has been reported for use in capture of human IL-10 by ELISA, intracellular flow cytometric analysis, and for neutralizing IL-10 bioactivity. (Use Functional Grade purified for functional studies cat.16-7108).

Applications Tested

The JES3-9D7 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-10 (IL-10) in combination with the biotin JES3-12G8 (13-7109) antibody for detection and recombinant human IL-10 (14-8109) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.

References

Nowacki TM, Kuerten S, Zhang W, Shive CL, Kreher CR, Boehm BO, Lehmann PV, Tary-Lehmann M. Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells. Cell Immunol. 2007 May;247(1):36-48. (JES3-9D7, ELISPOT, PubMed)

Sendide K, Deghmane AE, Pechkovsky D, Av-Gay Y, Talal A, Hmama Z. Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S. J Immunol. 2005 Oct 15;175(8):5324-32. (JES3-9D7, FA, PubMed)

Martin M, Schifferle RE, Cuesta N, Vogel SN, Katz J, Michalek SM. Role of the phosphatidylinositol 3 kinase-Akt pathway in the regulation of IL-10 and IL-12 by Porphyromonas gingivalis lipopolysaccharide. J Immunol. 2003 Jul 15;171(2):717-25. (JES3-9D7, NU in vitro, PubMed)

Chandler SW, Rassekh CH, Rodman SM, Ducatman BS. Immunohistochemical localization of interleukin-10 in human oral and pharyngeal carcinomas. Laryngoscope. 2002 May;112(5):808-15. (JES3-9D7, IHC paraffin)

Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.Immunol Rev. 1992 Jun;127:5-24.

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