Staining of C57BL/6 splenocytes with 0.5 µg of Rat IgG2a κ Isotype Control Purified (cat. 14-4321) (open histogram) or 0.5 µg of Anti-Mouse IgM Purified (filled histogram) followed by Anti-Rat IgG Biotin (cat. 13-4813) and Streptavidin PE (cat. 12-4317). Total viable cells were used for analysis.
Contents: Anti-Mouse IgM Purified Catalog Number: 14-5790 Concentration: 0.5 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 2-8°C. Clone: II/41 Host/Isotype: Rat IgG2a, κ
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Size
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14-5790-81
50 ug
14-5790-82
100 ug
14-5790-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The II/41 monoclonal antibody reacts with the μ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The II/41 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and ELISA.
Applications Tested
The II/41 antibody has been tested by flow cytometric analysis of mouse spleen cell suspension. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Laszlo G, Hathcock KS, Dickler HB, Hodes RJ. 1993. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J Immunol. 150(12):5252-62.