Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŪ 647 conjugated products with eFluorŪ 660 conjugated products.
Description
This antibody reacts with the heavy chains on Syrian hamster IgG and with light chains common to most Syrian hamster immunoglobulins. Reactions to non-immunoglobulin hamster proteins have not been detected.
Applications Reported
For research use only, not for diagnostic or therapeutic use. In conjunction with enzyme- or fluorochrome-conjugated Streptavidin (SAV) or Avidin (AV), this polyclonal antibody can be used for detection of purified Syrian hamster immunoglobulins (IgG) in ELISA, immunoblotting (WB), immunohistochemical staining and flow cytometry. At high concentrations, cross-reaction to Armenian hamster IgG has been observed.
Applications Tested
Syrian Hamster Isotype Control has been tested by flow cytometric analysis of mouse splenocyte suspensions. It should be used at the same concentration as the experimental antibody.