Contents: IC Fixation Buffer Catalog Number: 00-8222 Storage Conditions: Store at 2-8°C. Use within 6 months of receipt. Cautions: Harmful if inhaled, swallowed or by contact.
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00-8222-49
125 ml
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŪ 647 conjugated products with eFluorŪ 660 conjugated products.
Description
eBioscience Intracellular (IC) Fixation Buffer, used in conjunction with eBioscience Permeabilization Buffer (Cat. No. 00-8333), can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens. These solutions have been specially formulated for reducing non-specific staining by fluorochrome-labelled antibodies and increasing signal to noise ratios. For a more detailed protocol of intracellular staining for mouse and human cytokines, see: http://www.ebioscience.com/ebioscience/appls/FCI.htm.
The Fixation Buffer is used first in this protocol followed by Permeabilization Buffer. All washing steps, antibody dilutions, and incubations after cell permeabilization should be performed using the Permeabilization Buffer.
Applications Reported
This solution is at 1X working concentration and contains 4% Paraformaldehyde, which is toxic and suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided.