Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŪ 647 conjugated products with eFluorŪ 660 conjugated products.
Description
Monensin is an inhibitor of intracellular protein transport. Incubation of cells in culture with Monensin leads to blockade of protein transport to the Golgi complex (GC) and accumulation of proteins in the endoplasmic reticulum (ER). Addition of Monensin during the last hours of in vitro activation of cells results in enhanced detection of intracellular cytokines. Monensin is effective for enhanced detection of a majority of mouse and human intracellular cytokines; however, it is advised that the investigators evaluate the use and efficacy of this reagent as well as other protein transport inhibitors such as Brefeldin A in their specific assay system.
For research use only, not for diagnostic or therapeutic use. The Monensin Solution is supplied at 1000X working concentration. Prior to addition to the cell cultures, a 1:1000 dilution in the culture media should be made. The final concentration of the monensin (at 1x) is 2 uM.