Also known as: 'Interleukin-7 Receptor alpha, IL-7Ra
RUO: For Research Use Only
Staining of C57BL/6 splenocytes with Anti-Mouse CD3e FITC (cat. 11-0031) and 0.125 µg of Rat IgG2a κ Isotype Control APC-eFluor® 780 (cat. 47-4321) (left) or 0.125 µg of Anti-Mouse CD127 APC-eFluor® 780 (right). Total viable cells were used for analysis.
Contents: Anti-Mouse CD127 APC-eFluor® 780 Catalog Number: 47-1271 Concentration: 0.2 mg/ml Formulation: aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly. Clone: A7R34 Host/Isotype: Rat IgG2a, κ
Product Options
Cat. No.
Size
Price
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47-1271-80
25 ug
47-1271-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
eFluor® products are not offered for sale or use in certain regions. Please contact an eBioscience representative for product availability.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity α subunit of the mouse IL-7 receptor. IL-7 receptor α chain is expressed by immature B cells in the bone marrow, double-negative (CD4-/CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+/CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.
Applications Reported
This A7R34 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This A7R34 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
APC-eFluor® 780 is a replacement for APC-Alexa Fluor® 750. APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Caution should be used when fixing samples stained with APC-eFluor® 780 as this may lead to degradation of the fluorochrome and loss of staining.
References
Sudo, T., S. Nishikawa, et al. 1993. Expression and function of the interleukin 7 receptor in murine lymphocytes. Proc Natl Acad Sci U S A 90(19): 9125-9.
Ohana M, Okazaki K, Oshima C, et al. 2001. A critical role for IL-7R signaling in the development of Helicobacter felis-induced gastritis in mice. Gastroenterology. Aug;121(2):329-36.
Okuno Y, Iwasaki H, Huettner CS, Radomska HS, Gonzalez DA, Tenen DG, Akashi K. 2002. Differential regulation of the human and murine CD34 genes in hematopoietic stem cells. Proc Natl Acad Sci U S A. Apr 30;99(9):6246-51.
Leithauser F, Meinhardt-Krajina T, et al. 2006. Foxp3-expressing CD103+ regulatory T cells accumulate in dendritic cell aggregates of the colonic mucosa in murine transfer colitis. Am J Pathol. 168(6):1898-909. (A7R34, IHC frozen, PubMed)
LTbetaR signaling induces cytokine expression and up-regulates lymphangiogenic factors in lymph node anlagen.
Vondenhoff MF, Greuter M, Goverse G, Elewaut D, Dewint P, Ware CF, Hoorweg K, Kraal G, Mebius RE.
J Immunol. 2009 May 1;182(9):5439-45. (A7R34, IF, PubMed)