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Anti-Mouse IFN gamma PE-Cy7

Also known as: Interferon-gamma, IFN-g, IFNg

RUO: For Research Use Only

mouse IFN gamma antibody, Phycoerythrin-Cy7 (PE-Cy7) conjugate

Intracellular staining of Mouse Cytokine Positive Control Cells (cat. 00-4500) with Anti-Mouse CD4 APC (cat. 17-0041) and 0.25 µg of Rat IgG1 κ Isotype Control PE-Cy7 (cat. 25-4301) (left) or 0.25 µg of Anti-Mouse IFNγ PE-Cy7 (right).

Contents: Anti-Mouse IFN gamma PE-Cy7
Catalog Number: 25-7311
Concentration: μg size: 0.2 mg/ml; test size: 5 μl (0.25 μg)/test
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Clone: XMG1.2
Host/Isotype: Rat IgG1, κ

Product Options
Cat. No.	Size	Price	Add Qty to Cart
25-7311-41	25 tests	To view relevant pricing, please select your country and resume browsing.
25-7311-82	100 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
00-8222	IC Fixation Buffer
00-8333	Permeabilization Buffer (10X)
14-8311	Mouse IFN gamma Recombinant Protein
25-4301	Rat IgG1 K Isotype Control PE-Cy7
88-7234	Mouse IL-23 ELISA Ready-SET-Go!®
88-8823	Fixation & Permeabilization Buffers

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
11-7311	Anti-Mouse IFN gamma FITC	488	518	IC Flow	RUO
12-7311	Anti-Mouse IFN gamma PE	488	575	IC Flow	RUO
13-7311	Anti-Mouse IFN gamma Biotin	N/A	N/A	ELISA	RUO
14-7311	Anti-Mouse IFN gamma Purified	N/A	N/A	ELISA cap	RUO
16-7311	Anti-Mouse IFN gamma Functional Grade* Purified	N/A	N/A	NU	RUO
17-7311	Anti-Mouse IFN gamma APC	633	660	IC Flow	RUO
19-7311	Anti-Mouse IFN gamma Cy5	633	670	IC Flow	RUO
25-7311	Anti-Mouse IFN gamma PE-Cy7	488	760	IC Flow	RUO
45-7311	Anti-Mouse IFN gamma PerCP-Cy5.5	488	690	IC Flow	RUO
48-7311	Anti-Mouse IFN gamma eFluor® 450 (Pacific Blue® replacement)	405	450	IC Flow	RUO
51-7311	Anti-Mouse IFN gamma Alexa Fluor® 647	633	668	IC Flow	RUO
53-7311	Anti-Mouse IFN gamma Alexa Fluor® 488	488	519	IC Flow	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

The XMG1.2 antibody reacts with mouse interferon-γ. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN-γ is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN-γ, in synergy with other cytokines such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This XMG1.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

This XMG1.2 antibody is offered in 2 formats:

- μg size: has been tested by intracellular staining and flow cytometric analysis of ConA-stimulated mouse splencoytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

- test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of ConA-stimulated mouse splencoytes. This can be used at test size: 5 μl (0.25 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. The pre-titrated test size contains BSA and/or gelatin for protein stabilization.

References

Kimura A, Naka T, Kishimoto T. IL-6-dependent and -independent pathways in the development of interleukin 17-producing T helper cells. Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12099-104. (XMG1.2, IC flow, PubMed)

Zhang Y, Xu G, Zhang L, Roberts AI, Shi Y. Th17 cells undergo Fas-mediated activation-induced cell death independent of IFN-gamma. J Immunol. 2008 Jul 1;181(1):190-6. (XMG1.2, IC flow, PubMed)

Cho KS, Hill AB. T cell acquisition of APC membrane can impact interpretation of adoptive transfer experiments using CD45 congenic mouse strains. J Immunol Methods. 2008 Jan 31;330(1-2):137-45. (XMG1.2, IC flow, PubMed)

Feng X, Akiyoshi DE, Sheoran A, Singh I, Hanawalt J, Zhang Q, Widmer G, Tzipori S. Serial propagation of the microsporidian Enterocytozoon bieneusi of human origin in immunocompromised rodents. Infect Immun. 2006 Aug;74(8):4424-9. (XMG1.2, FA)

Hidalgo LG, Urmson J, Halloran PF. IFN-gamma decreases CTL generation by limiting IL-2 production: A feedback loop controlling effector cell production. Am J Transplant. 2005 Apr;5(4 Pt 1):651-61. (XMG1.2, NU, PubMed)

Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992 Jun;127:5-24.

Legal

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT # 5,268,486, 5,569,587 AND 5,627,027 AND FOREIGN EQUIVALENTS AND PENDING APPLICATIONS.

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