Contents: Anti-Mouse/Rat IL-1 beta Functional Grade Purified Catalog Number: 16-7012 Concentration: 1 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, No NaN3 Storage Conditions: Store at 2-8°C. Handling Conditions: Use in sterile envrioment. Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay. Clone: B122 Host/Isotype: Armenian Hamster IgG
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16-7012-81
50 ug
16-7012-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The B122 antibody reacts with mouse and rat interleukin-1β (IL-1β), a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The B122 antibody has been reported for use in cytokine neutralization and ELISA capture.
Applications Tested
The Functional Grade Purified B122 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-1β bioactivity.
The B122 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-1 beta (IL-1β) in combination with the biotin anti-mouse Interleukin-1 beta (IL-1β) polyclonal (13-7112) antibody for detection and recombinant mouse IL-1β (14-8012) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml – 15 pg/ml should be included in each ELISA plate.
References
Hogquist, K, et al. 1991. Generation of monoclonal antibodies to murine IL-1beta and demonstration of IL-1 in vivo. J. Immunol. 146: 1534-1540. Rogers, H.W., et al. 1992. Interleukin 1 participates in the development of anti-Listeria responses in normal and SCID mice. Proc Natl Acad Sci U S A. 89: 1011-5.