Mouse IL-1α ELISPOT Assay: Mouse peritoneal exudate cells were elicited with thioglycolate, harvested, and stimulated with 1 µg/ml LPS for 24 hrs. Control well is medium alone.
Contents: Anti-Mouse IL-1 alpha Functional Grade Purified Catalog Number: 16-7011 Concentration: 1 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, No NaN3 Storage Conditions: Store at 2-8°C. Handling Conditions: Use in sterile envrioment. Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay. Clone: ALF-161 Host/Isotype: Armenian Hamster IgG
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16-7011-81
50 ug
16-7011-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The ALF-161 antibody reacts with mouse interleukin-1α (IL-1α). Mouse IL-1α, also called Lymphocyte Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), and Mononuclear Cell Factor (MCF), is a 17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1α is mostly cell-associated, with 23% amino acid homology with IL-1β. The immune regulatory role of IL-1α is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The ALF-161 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization, and intracellular staining for flow cytometry.
Applications Tested
The Functional Grade Purified ALF-161 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-1α bioactivity.
The ALF-161 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-1 alpha (IL-1α) in combination with the biotin anti-mouse Interleukin-1 alpha (IL-1α) polyclonal (13-7111) antibody for detection and recombinant mouse IL-1α (14-8011) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000pg/ml - 8pg/ml should be included in each ELISA plate.
References
Fuhlbrigge, R., et al. 1988. Monoclonal antibodies to murine IL-1alpha. J. Immunol. 141: 2643-50. Rogers, H.W., et al. 1992. Interleukin 1 participates in the development of anti-Listeria responses in normal and SCID mice. Proc Natl Acad Sci U S A. 89: 1011-5.