Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŪ 647 conjugated products with eFluorŪ 660 conjugated products.
Description
The polyclonal antibody reacts with both mouse and rat CXCL12 β (SDF-1β) and was generated using E. coli-expressed mouse CXCL12 β as an immunogen. CXCL12 β, also known as pre-B-cell growth-stimulating factor (PBSF) and stromal cell-derived factor-beta (SDF-1β), is a 74-amino acid CXC chemokine originally cloned from a bone marrow stromal cell line. Targeted deletion of CXCL12 gene resulted in defects of B-cell lymphopoiesis and bone marrow myelopoiesis in mice. CXCL12 has been shown to be chemotactic for lymphocytes. In addition, CXCL12 was recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV-1 entry into T cells. CXCL12 binding to CXCR4 inhibits HIV-1 entry.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal can be used in immunoblotting (WB), immunoprecipitation, and immunohistochemistry. Cross-reactivity to CXCL12 β of other species has not been determined.
Applications Tested
To achieve best results, titrate antibody by dilution for each application as follows: Western Blot 1:1000 Immunoprecipitation 1:300-500 Immunohistochemistry 1:100-500.
References
Tashiro, K. et al. 1993. Signal sequence trap: a cloning strategy for secreted proteins and type I membrane proteins. Science 261:600-603. Nagasawa, T et al. 1994. Molecular cloning and structure of a pre-B-cell growth-stimulating factor. Proc. Natl. Acad. Sci. USA 91:2305-2309. Nagasawa, T. et al. 1996. Defeats of B-cell lymphopoiesis and bone-marrow myelopoiesis in mice lacking the CXC chemokine PBSF/SDF-1. Nature 328:635-638. Bleul. C.C. et al. 1996. A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1). J. EXP. Med. 184:1101-1109. Bleul. C.C. et al. 1996. The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry. Nature 382:829-833. Oberlin E. et al. 1996. The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by C-cell-line-adapted HIV-1. Nature 382:833-835.