Also known as: Tumor Necrosis Factor alpha, TNFa TNF-a
RUO: For Research Use Only
Mouse splenocytes were stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized Anti-Mouse CD3 Functional Grade Purified (cat. 16-0031) and soluble Anti-Mouse CD28 Functional Grade Purified (cat. 16-0281) in the presence of Brefeldin A for 5 hours. The cells were surface stained with Anti-Mouse CD4 FITC (cat. 11-0041) and intracellularly stained with Anti-Mouse TNFα PE. (right). The cells were surface stained with Anti-Mouse CD4 PE (cat 12-0041) and intracellularly stained with Anti-Mouse TNF α APC.
Contents: Anti-Mouse TNF alpha Purified Catalog Number: 14-7321 Concentration: 0.5 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 2-8°C. Clone: MP6-XT22 Host/Isotype: Rat IgG1, κ
Product Options
Cat. No.
Size
Price
Add Qty to Cart
14-7321-81
50 ug
14-7321-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF-α), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF-α has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF-α forms dimers and trimers and also exists as a 26 kDa membrane-bound form.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This MP6-XT22 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and immunohistology staining of frozen tissue sections. (Fluorochrome conjugated MP6-XT22 is recommended for use in intracellular flow cytometry.)
Applications Tested
The MP6-XT22 antibody has been tested to block staining with fluorochrome conjugated MP6-XT22. This can be used at less than or equal to 2 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Hunter CA, Litton MJ, et al. 1994. Immunocytochemical detection of cytokines in the lymph nodes and brains of mice resistant or susceptible to toxoplasmic encephalitis. J Infect Dis. 170(4): 939-45.
Litton MJ, Sander B, et al. 1994. Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B. J Immunol Methods 175(1): 47-58.
Abrams JS, Roncarolo MG, et al. 1992. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 127: 5-24.
Chackerian B, Lowy DR and Schiller JT. 2001. Conjugation of a self-antigen to papillomavirus-like particles allows for efficient induction of protective autoantibodies. J Clin Invest. 108(3):415-23. (IHC frozen, PubMed)
Williams RO, Mauri C, et al. 1998. Therapeutic actions of cyclosporine and anti-tumor necrosis factor alpha in collagen-induced arthritis and the effect of combination therapy. Arthritis Rheum. 41(10):1806-12. (IHC frozen, PubMed)